Journal of Ultrasound in Medicine, Vol 10, Issue 11 629-636, Copyright © 1991 by American Institute of Ultrasound in Medicine
Method of cavitation-suppressed exposure of cells and explant mouse embryos to clinical real-time and pulsed Doppler ultrasound
E. L. Madsen, G. R. Frank, M. C. Macdonald, A. O. Martin, N. P. Bouck and P. M. Iannaccone
Department of Medical Physics, University of Wisconsin, Madison 53706.
An apparatus and procedure for well-controlled exposure of cells or explant
mouse embryos to clinical real-time ultrasound are described. Cells or
embryos to be exposed are suspended in media made sufficiently viscous
through inclusion of methylcellulose that cavitation is suppressed but
thermal effects remain negligible. During exposure, the scanning beam is
precisely centered in a 2 mm x 20 mm slot in a 20 cm diameter agar disc
containing the suspension. The high viscosity causes the cells to remain
distributed uniformly throughout the exposure; this fact, along with
precision beam alignment, ensures that exposure is well defined. Exposure
data are acquired with a 0.6 mm diameter hydrophone.
